Cell Biolabs彗星实验试剂盒

由于环境因素和细胞内的正常代谢过程造成的DNA损伤,每个细胞每天都会发生1,000到1,000,000个。虽然这些只占人类基因组约60亿个碱基中的一小部分,但如果关键基因损伤未及时修复可能会阻碍细胞的正常生理功能,进而增加癌变可能。彗星实验,或称单细胞凝胶电泳(Single cell gel electrophoresis,SCGE是一种测量单个细胞DNA损伤的常用技术。其原理简单,即在电泳场,将受损细胞DNA(包含片段和链断裂)与完整的DNA分离通过显微镜观察到损伤细胞呈现出典型的彗星状尾巴,然后通过测量计算彗尾大小对比出细胞DNA损伤的程度因为彗星实验特点,该方法几乎被用来评估任何类型的真核细胞的 DNA 修复能力,包括双、单链断裂的不同的 DNA 损伤情况。是一种能快速、大通量检测真核细胞DNA损伤进而判别遗传毒性的技术。


彗星实验原理简单,但操作繁琐,需要丰富的实验经验和技巧,尤其常常出现的“脱胶”问题,困扰了科研人员除此之外有时为了跑出完美的“彗星”图案放在paper里,还需要重复做许多次实验,费时费力。为了解决上述问题,我们推荐CellBiolabsOxiSelectTMComet Assay Kit即彗星实验试剂盒来检测细胞的DNA损伤。该试剂盒不仅能让彗星实验化繁为简,还两种不同规格(3孔和96孔)的细胞电泳凝胶板供选择让少量样本和大量样本的DNA损伤检测通通轻松hold住。用该试剂做彗星实验流程如下图。

除了操作简便,OxiSelectTMComet Assay Kit还有以下优点:

1)      适用于各种DNA损伤检测,是一款非常好用的DNA损伤检测筛选工具;

2)      试剂盒中的载玻片经过特殊处理以粘附低熔点琼脂糖,避免“脱胶”问题出现

3)      采用特殊的DNA荧光染料,能有效降低背景干扰,更加方便读取实验结果。






OxiSelectTM Comet Assay Kit (3-Well Slides)


15 assays


OxiSelectTM Comet Assay Kit (3-Well Slides)


75 assays


OxiSelectTM Comet Assay Kit (96-Well Slides)


96 assays








Comet Assay Slides, 3-Well


5 slides


25 slides

Comet Assay Slides, 96-Well


1 slides


5 slides


Maiuri, T. et al. (2016). Huntingtin is a scaffolding protein in the ATM oxidative DNA damage response complex. Hum. Mol. Genet. doi:10.1093/hmg/ddw395.

Irianto, J. et al. (2016). Nuclear constriction segregates mobile nuclear proteins away from chromatin. Mol. Bio. Cell doi:10.1091/mbc.E16-06-0428.

Andronescu, E. et al. (2016). Nanomaterials for medical applications: Benefits and risks. J Nanomater. doi:10.1155/2016/8284319.

Liu, Z. et al. (2016). Canonical microRNAs enable differentiation, protect against DNA damage, and promote cholesterol biosynthesis in neural stem cells. Stem Cells and Dev. doi:10.1089/scd.2016.0259.

Dai, C. et al. (2016). Curcumin ameliorates furazolidone-induced DNA damage and apoptosis in human hepatocyte L02 cells by inhibiting ROS production and mitochondrial pathway. Molecules. 21:1061.

Beegle, J. R. et al. (2016). Preclinical evaluation of mesenchymal stem cells overexpressing VEGF to treat critical limb ischemia. Mol Ther Methods Clin Dev.doi:10.1038/mtm.2016.53.

Suzuki, Y. et al. (2016). Pharmacodynamics of anti-inflammatory drugs–intranasal corticosteroid and dna damage. Pharmacodynamics of anti-inflammatory drugs. 117-126.

Zhang, J. et al. (2016). Inhibition of Glucose-6-Phosphate Dehydrogenase could Enhance 1, 4-Benzoquinone-induced Oxidative Damage in K562 Cells. Oxid Med Cell Longev. doi:10.1155/2016/3912515.

Jang, J. H. et al. (2016). APO-9′-fucoxanthinone extracted from undariopsis peteseniana protects oxidative stress-mediated apoptosis in cigarette smoke-exposed human airway epithelial cells. Mar Drugs. doi:10.3390/md14070140.

Ebeid, S. A. et al. (2016). Assessment of the radioprotective effect of propolis in breast cancer patients undergoing radiotherapy. New perspective for an old honey bee product. J Radiat Res Appl Sci. doi:10.1016/j.jrras.2016.06.001.

Wang, H, & Kim, N. H. (2016). CDK2 is required for the DNA damage response during porcine early embryonic development. Biol Reprod. doi:10.1095/biolreprod.116.140244.

Zhao, X. et al. (2016). Dioscin induces apoptosis in human cervical carcinoma HeLa and SiHa cells through ROS-mediated DNA damage and the mitochondrial signaling pathway. Molecules. doi:10.3390/molecules21060730.

Chen, X. et al. (2016). Zidovudine, abacavir and lamivudine increase the radiosensitivity of human esophageal squamous cancer cell lines. Oncol Rep. 36:239-246.

Coleman, J. et al. (2016). Detecting Apoptosis, Autophagy, and Necrosis. Apoptosis Methods in Toxicology . doi:10.1007/978-1-4939-3588-8_5.

Ding, Y. et al. (2016). Induction of ROS overload by alantolactone prompts oxidative DNA damage and apoptosis in colorectal cancer cells. Int J Mol Sci. doi:10.3390/ijms17040558.

Cui, F. M. et al. (2016). The role of miR-34a in tritiated water toxicity in human umbilical vein endothelial cells. Dose-Response. doi:10.1177/1559325816638585.

Laks, D. R. et al. (2016). Inhibition of nucleotide synthesis targets brain tumor stem cells in a subset of glioblastoma. Mol Cancer Ther. doi:10.1158/1535-7163.MCT-15-0982.

Abubakar, I. B. et al. (2016). Synergistic cytotoxic effects of combined δ-tocotrienol and jerantinine B on human brain and colon cancers. J Ethnopharmacol. doi:10.1016/j.jep.2016.03.004.

Hofstetter, C. et al. (2016). Inhibition of KDM6 activity during murine ESC differentiation induces DNA damage.J Cell Sci. 129:788-803.

Si, L. et al. (2016). Dioscin suppresses human laryngeal cancer cells growth via induction of cell-cycle arrest and MAPK-mediated mitochondrial-derived apoptosis and inhibition of tumor invasion. Eur J Pharmacol. doi:10.1016/j.ejphar.2016.02.009.

Qu, L. et al. (2016). Corosolic acid analogue, a natural triterpenoid saponin, induces apoptosis on human hepatocarcinoma cells through mitochondrial pathway in vitro. Pharm Biol. doi:10.3109/13880209.2015.1104699.

Singh, A. K. et al. (2015). Parental age affects somatic mutation rates in the progeny of flowering plants. Plant Physiol. doi:10.1104/pp.15.00291.

Yuan, L. et al. (2015). Serum collected from fruit and vegetable juice treated rats antagonizing H2O2-induced oxidative damage in PC12 cells. J Funct Foods. 20:496-505.

Ramy, N. et al. (2015). Jaundice, phototherapy and DNA damage in full-term neonates.  J Perinatol.  doi:10.1038/jp.2015.166.

Wu, C. F. et al. (2015). Anticancer activity of cryptotanshinone on acute lymphoblastic leukemia cells. Arch Toxicol. doi:10.1007/s00204-015-1616-4.

Kim, M. J. et al. (2015). Antibacterial effect and mechanism of high-intensity 405±5nm light emitting diode on Bacillus cereus, Listeria monocytogenes, and Staphylococcus aureus under refrigerated condition. J Photochem Photobiol B. 153:33-39.

Wang, X. et al. (2015). Enhancement of arabinocytosine (AraC) toxicity to AML cells by a differentiation agent combination. J Steroid Biochem Mol Biol.  doi:10.1016/j.jsbmb.2015.08.023.

Sun, X. et al. (2015). Electrochemical detection of 8-hydroxy-2′-deoxyguanosine as a biomarker for oxidative DNA damage in HEK293 cells exposed to 3-chloro-1, 2-propanediol. Anal Methods.doi:10.1039/C5AY01246E.

Neumann, J. et al. (2015). Mangrove dolabrane‐type of diterpenes tagalsins suppresses tumor growth via ROS‐mediated apoptosis and ATM/ATR–Chk1/Chk2‐regulated cell cycle arrest. Int J Cancer.  doi: 10.1002/ijc.29629.

Singh, A. K. et al. (2015). Parental age affects somatic mutation rates in the progeny of flowering plants. Plant Physiol. 168:247-257.

Hou, W. et al. (2015). The protecting effect of deoxyschisandrin and schisandrin B on HaCaT cells against UVB-induced damage. PLoS One. 10:e0127177.

Kim, M. J. et al. (2015). Inactivation by 405±5 nm light emitting diode on Escherichia coli O157: H7, Salmonella Typhimurium, and Shigella sonnei under refrigerated condition might be due to the loss of membrane integrity. Food Control. doi:10.1016/j.foodcont.2015.05.012.

Haeger, S. M. et al. (2015). Smad4 loss promotes lung cancer formation but increases sensitivity to DNA topoisomerase inhibitors. Oncogene.  doi: 10.1038/onc.2015.112.

Ong, J. Y. et al. (2015). 2-Methoxy-1, 4-naphthoquinone (MNQ) induces apoptosis of A549 lung adenocarcinoma cells via oxidation-triggered JNK and p38 MAPK signaling pathways. Life Sci. doi: 10.1016/j.lfs.2015.03.019.

Prasad, M. A. et al. (2015). Ebf1 heterozygosity results in increased DNA damage in pro-B cells and their synergistic transformation by Pax5 haploinsufficiency. Blood.  doi: 10.1182/blood-2014-12-617282.

Obstoy, B. et al. (2015). Safety and performance analysis of acriflavine and methylene blue for in vivo imaging of precancerous lesions using fibered confocal fluorescence microscopy (FCFM): an experimental study. BMC Pulm Med. 15:30.

Xiong, J. et al. (2015). Stemness factor Sall4 is required for DNA damage response in embryonic stem cells.J Cell Biol. 208:513-520.

Hu, Y. et al. (2015). Bile acids regulate nuclear receptor (nur77) expression and intracellular location to control proliferation and apoptosis. Mol Cancer Res. 13:291-292.

Gong, L. et al. (2015). p53 isoform Δ113p53/Δ133p53 promotes DNA double-strand break repair to protect cell from death and senescence in response to DNA damage. Cell Res. 25:351-369. 

Jin, L. et al. (2015). Association between oxidative DNA damage and the expression of 8-oxoguanine DNA glycosylase 1 in lung epithelial cells of neonatal rats exposed to hyperoxia. Mol Med Rep. doi: 10.3892/mmr.2015.3339.

北京西美杰科技有限公司是Cell Biolabs品牌全国一级代理,为用户提供完善的技术支持与售后服务。如对产品感兴趣欢迎拨打西美杰客服热线400-050-4006或了解更多信息。


网站地图 金沙赌城开户登入 威尼斯游戏平台登入 澳门新金沙在线开户登入
申博太阳城娱乐18k 申博亚洲官 太阳城电子游戏网址 申博太阳城官方网
新濠天地众乐博担保 威尼斯人上搜博网登入 拉斯维加斯AG电子 亚洲星ag馆直营网
金沙娱乐sands官网登入 鑫汇商城购物平台登入 金沙娱乐平台官网登入 合肥国鑫汇娱乐会所登入
金沙网上娱乐登入 恒和娱乐登入 鑫汇国际平台登入 威尼斯人娱乐平台登入
XSB599.COM 5888DZ.COM 998XTD.COM XSB698.COM 196psb.com
998jbs.com 711PT.COM XSB594.COM 1112125.COM 116DC.COM
686jbs.com 111xsb.com 44sbsg.com 618cw.com 156tt.com
18csb.com 304psb.com 66sbmsc.com 789XTD.COM 77TGP.COM